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procedure, all animals survived for the duration of the study.
Viral plaque assays. Persistent RSV infection was documented by positive viral Saucony Bait Cruel World 6
plaque assays of lung homogenates in all groups of animals that received virus. No RSV was isolated from the lungs of any of the animals within the sham inoculated CH (n 12) or STR2 (n 10) groups. Semiquantitative RT PCR. Figure 2 summarizes the results o.
Saucony Courageous Grey Group comparisons of mean PC2, titers of intrapulmonary replicating RSV, lung IFN IL 5/ IFN mRNA ratios and the percentage of the airway wall occupied Saucony Public Gardens
s was calculated as follows: [positive points/(total number of grid points in AOI luminal points)] 100. For each animal, the results for individual airways were averaged and expressed as the percentage of airway wall occupied by T cells or eosinophils (21). Data analysis. For all analyses, SPSS v. 11 (Statistical Products and Services Solutions, Chicago, IL) software was used, and, when warranted, data underwent logarithmic transformation to better approximate a normal distribution (16). For the ACh challenge, dose response curves for the experimental groups were compared using general linear model with repeated measures and analysis of variance (ANOVA), followed by t tests for pairwise group comparisons at individual ACh doses (14,29). The provocative concentration of ACh associated with a doubling of the value of baseline RL (PC2) was determined from each animal's ACh dose response curve (29).
by T cells and eosinophils were performed using ANOVA. Whenever significant differences (p 0. 05) were found by ANOVA, groups underwent further pairwise comparisons using t tests, with a Bonferroni procedure used to account for multiple comparisons (30). In all analyses, a two tailed p value was considered to be statistically significant. Top of pageRESULTSDuring the experiments, animals tolerated all CpG ODN and sham inoculations. With the exception of one Cam Hartley guinea pig that completed a full course CpG ODN immunoprophylaxis but died during the subsequent RSV inoculation Saucony Omni 12
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