donderdag, 31 mei 2012

Saucony Usa Made

The same data as in Fig.

3a, except genes were classified based on whether their 3 UTR contained no CPE (grey), one CPE (blue), or two or more non overlapping CPEs (red). b, Evidence that the more restricted tail length range observed at gastrulation did not substantially impact the coupling between tail length and translational efficiency. The zebrafish 4 data from Fig. 3a were sampled with replacement so as to have the same distribution of tail lengths observed at 6 (left). Likewise, the X. laevis stage 9 data were sampled with replacement so as to have the same distribution of tail lengths observed at stage 12 (right).

Saucony Usa Made

Saucony Usa Made

in Fig. 4b for the same set of genes, with slopes calculated omitting data from the fraction without bound ribosomes. d, Box plots as in Fig. 4b, creating four equal bins of genes based on either overall mean poly(A) tail length (left) or translational efficiency (right). The same slopes were used as in Fig. a, The relationship between changes in tail length at 4 (as determined by PAL seq) and changes in mRNA abundance at 6 (as determined by RNA seq), after injecting miR 155 (left) or miR 132 (right).

Because injected miRNAs partially inhibited miR.

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Saucony Usa Made

c, Box plot as Saucony Burger Shoes

Saucony Usa Made

Saucony Usa Made

Saucony Usa Made

genes from which controls were chosen, two adjacent isoforms were picked randomly. P value, Fisher exact test, comparing genes with a CPE in the unique region to controls. a, Saucony Extra Butter

 in the unique region to be counted as present, a canonical poly(A) signal (AAUAAA) also had to exist in the last 30 nucleotides of the distal isoform19, 24. Poly(A) tags from three zebrafish 2 PAL seq libraries (mock , miR 132 and miR 155 were combined before calculating average tail length for each isoform. Tandem isoform pairs with a target site for miR 132 or miR 155 in the region unique to the distal isoform were not considered. For genes for which isoform choice affected inclusion of a CPE, the isoform pair representing that gene was chosen as the two Saucony Usa Made isoforms with the most 5 poly(A) sites that flanked a CPE and satisfied the above criteria. For the pool of Saucony Shadow 5000 Grey

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Saucony Usa Made

Saucony Usa Made

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